The tunes, them selves, don't deteriorate. Especially when on the dyno both times. (pesky AFVs would have been zeroed out for the runs).
How many miles, between runs?
and... Tsani's questions... (good questions BTW)
Same dyno?
Same operator?
The miles ARE important, as when ring seal, etc start losing its effectiveness, the power will drop some. 2-3000 miles difference, one shouldn't see this. 15-20,000 yes, it can happen.
If you have BOTH sheets, the run conditions can tell the story. Was one run at 1.01 or .99 SAE correction factor? Then the next run at like a 1.04 factor? Makes a difference.
Think of it this way. You have WORD on your PC. And the PC has been running well for five years. Do word documents change themselves in that amount of time? No? Same with the tune, for the most part, and the parts that WILL change (meant to do so) will be set back to ZERO if another dyno run is made.